The Effect of High Mobility Group Box 1 on Cell Cycle Distribution and the Expression of Cell Cycle Protein in Mouse Mesangial Cell

Abstract: At this study we detected the distribution of cell cycle and the expression changes of PCNA， CyclinD1， CDK4 and p16 in MMC. We also analyze the relationship among them in order to explore the possible effect of HMGB1 on the generation of MMC. MMC were obtained from the Department of Medical Pathology of Hebei Medical University. MMC selected for the study were randomly divided into control group and HMGB1 stimulation group (0.05 mg/L). The cells were collected after 4， 8， 12 h. The changes in cells cycle distribution were detected by flow cytometry. Immunocytochemical stain were used to detect the over expression of PCNA protein in MMC; the level of CyclinD1 mRNA were detected by RT-PCR; Western blot detected that HMGB1 up-regulated the levels of CDK4 protein and the CyclinD1 protein in MMC， decreased the expression of p16 protein in MMC. We can see that: HMGB1 could induce mouse mesangial cells proliferation and promote the transition of cell cycle from G1 stage to S stage by up-regulating CyclinD1/CDK4/p16 pathway， which might be an effect mechanism of HMGB1 in lupus nephritis pathogenesis.

CSTR: 32200.14.cjcb.2011.11.0007