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The Correlation Between Phenotypes of Human Peripheral Blood Lymphocytes Induce-cultured in vitro and Cytotoxic Activity
Yun-Hong Liu, Ming-Yao Meng, Yan-Hua Xie, Ying Liu, Chuan-Yu Wei, Zong-Liu Hou*
Key Lab of Kunming Medical College Affiliated Yan’an Hospital, Kunming 650051, China
Abstract: In order to analyze the correlation between the phenotypes of in vitro cultured CIK cells and the cytotoxic activity， normal human peripheral blood lymphocytes and mononuclear cells were cultured and the cell percentage variation of 15 different phenotypes was measured by flow cytometry at the culture time points 0， 7 and 14 days. In addition， the cytotoxic activity was tested with CCK-8 reagent. The results indicated that the expression of T cell active phenotypes after amplification and its cytotoxic activity peaked at the 7th day. there is positive correlation (P<0.05) with cell phenotype CD3+CD25+， CD3+CD28+， CD3+CD25+CD28+， CD3+CD4+ and negative correlation (P<0.05) with CD3+CD45RA+CD45RO+. Thus， our study shows that determination of related phenotype of activated cells can indirectly monitor their cytotoxic activity to tumor， providing experimental basis for clinical application of adoptive immunotherapy with CIK cells.
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