Distinct Effects of OMgp Domains in Neurite Outgrowth Inhibition

Abstract: The oligodendrocyte-myelin glycoprotein (OMgp)， a phosphatidylinositol-linked membrane glycoprotein expressed in the brain， was found to be an inhibitor of neurite outgrowth and axonal regeneration after brain injury. Several observation suggest that the LRR domain of OMgp may play an important role in inhibition of cell proliferation. Here we report an analysis of the domain requirement of OMgp in neurite outgrowth inhibition .The distinct domain of OMgp was subcloned into pGEX-4T vector and GST-fusion protein comprised distinct domain of OMgp were expressed. The protein were used to culture with NgR-expressing CHO and hippocampal neurons. Results show that the fragment consisting of the S/T domain and C-terminal of OMgp can not bind with NgR-expressing CHO cell. The fragment representing of LRR can bind with NgR-expressing CHO and inhibit the neurite outgrowth of primary neurons. It can also pull down NgR from mouse brain. The results suggest that the LRR domain of OMgp is required and sufficient for binding of NgR and neurite outgrowth inhibition.