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The Optimization of Cell Suspensiong Culture Protocol of Lavandula angustifolia， cv. Munstead
Yao-Zu Xu 1,2,3, Xiao-Jun Wang1*, Min-An Zhao 1, Yan-Yu Wei 1, Hai-Qing Zhao 1 , Kan Lin 1
1Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi 830011, China;2Graduate School of Chinese Academy of Sciences, Beijing 100830, China;3Zhejiang CIFU Biotechnology INC, Hangzhou 3
Abstract: By the orthogonalty design， the factors influencing suspension cells growth of L.angustifolia were studied， and best culture conditions which were B5 media supplemented with 40 g/L sucrose， 0.025 mg/L 2，4-D， 0.5 mg/L 6-BA and the rotating speed of 120 r/min were sifted. After 15 days of culture， the density of surviving cells could reach 4.4×105 cell/ml， and differentiation rate of suspension cells could surpass 90% after 3 months of suspension culture. The cause of effects of sucrose concentration on the density of surviving cells was discussed through the analysis of the consumption rule of surplus sucrose.
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