Application and Exploration of C2C12 Cell Induced Differentiation and Comprehensive Cytological Detection Technology in Undergraduate Cell Biology Experiment Teaching
Wuyundalai, Soyolt, Meirong*
To improve the scientific thinking and practical research abilities of undergraduate biology students, this study designed an inquiry-based experiment centered on the induced differentiation of C2C12 cells, based on the prior foundation of Cell Biology experimental courses. The experiment integrates multiple techniques, including cell recovery, subculture, induced differentiation, immunofluorescence staining, Giemsa staining, inverted microscopy, and confocal laser scanning microscopy. C2C12 cells were induced to differentiate into myocytes, and their differentiation characteristics were confirmed through combined staining approaches. Teaching practice demonstrated that this experimental module effectively integrates previously fragmented experimental knowledge and skills, helps students establish systematic scientific thinking, and improves their comprehensive experimental competence and scientific inquiry ability, thereby laying a solid foundation for subsequent academic exploration and research practice.
CN
EN