The Effect of LncRNA FGD5‑AS1 on the Malignant Biological Behaviors of Laryngeal Cancer Cells by Regulating miR‑22‑3p/ISG15 Expression
FAN Hao, QI Bin*
This study aims to investigate the effect of LncRNA FGD5-AS1 on the malignant biological behaviors of laryngeal cancer cells by regulating miR-22-3p/ISG15 expression. qRT-PCR was used to detect the mRNA expression levels of FGD5-AS1, miR-22-3p and ISG15 in laryngeal cancer tissues and cells. At the same time, the best cell line was selected for subsequent experiments. TU212 cells were assigned into the si-NC group, the FGD5-AS1 siRNA group, the miR-NC group, the miR-22-3p group, the FGD5-AS1 siRNA+anti-miR-22-3p group, and the FGD5-AS1 siRNA+ISG15 group. The untreated cells were used as the blank group. qRT-PCR was used to detect the expression levels of FGD5-AS1, miR-22-3p and ISG15 mRNA; the dual-luciferase reporter gene assay and RIP (RNA immunoprecipitation) experiment were used to verify the targeting relationship of miR-22-3p with FGD5-AS1 and ISG15, respectively; CCK-8 and EdU assays were used to detect cell proliferation; Transwell and scratch assays were used to examine cell invasion and migration; Western blot was used to detect the expression levels of c-Myc, p21, ZO-1 (zonula occludens-1), and Vimentin. The dual luciferase reporter gene assay results showed that miR-22-3p had a targeting relationship with FGD5-AS1 and ISG15. The expression of FGD5-AS1 and ISG15 mRNA increased in laryngeal cancer cells and tissues, while the expression of miR-22-3p decreased (P<0.05). Knocking down FGD5-AS1 or overexpressing miR-22-3p could inhibit the proliferation, invasion and migration of TU212 cells (P<0.05); inhibiting the expression of miR-22-3p or overexpressing ISG15 could reverse the inhibitory effects of knocking down FGD5-AS1 on the proliferation, invasion and migration of TU212 cells (P<0.05); the LncRNA FGD5-AS1 is upregulated in laryngeal cancer cells. Interference with LncRNA FGD5-AS1 can inhibit the malignant biological behaviors of laryngeal cancer cells by regulating miR-22-3p/ISG15 expression.
CN
EN