Experimental Design for Quantitative Analysis of Membrane Protein Mobility Based on Fluorescence Recovery after Photobleaching
WANG Shuwei1, CUI Xiankui2, ZHU Lei1, WANG Xiangfeng1, FU Ying1*
FRAP (fluorescence recovery after photobleaching) is a crucial technique for quantifying plasma membrane fluidity and is widely used in the study of cell membrane dynamics. Vesicle trafficking, which includes processes such as endocytosis and exocytosis, represents a fundamental cellular activity essential for the exchange of substances between intracellular and extracellular compartments. Its function efficiency is closely tied to the f luidity of the plasma membrane. Guided by the principle of integrating research and education, this project intro duces an FRAP module into the “Cell Biology Experiment” course for graduate students, aiming to elucidate the intrinsic relationship between “vesicle trafficking” and “membrane fluidity”. The experimental teaching framework emphasizes FRAP’s unique capability to monitor real-time live-cell dynamics, while establishing a comprehensive training system that integrates “theoretical knowledge-experimental operation-data analysis”. This approach deep ens students’ understanding of core scientific concepts, such as membrane fluidity and vesicle trafficking, as well as FRAP; plasma membrane fluidity; vesicle trafficking; live-cell imaging.
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