Effects of High-Fat Diet-Induced Disturbance of Intestinal Flora on Female Reproduction in Mice
MA Yefei, LANG Ranran, ZHAO Limin, LIU Jun, WANG Jingjing*
Excessive obesity resulting from a high-fat dietary regimen is a predominant factor contributing to the decline in female reproductive capacity. Research findings indicate that obesity of this nature results in disruption of the gut microbiota. Nevertheless, the question of whether obesity-induced alterations in gut microbiota influence female fertility remains unresolved. To explore this issue, four murine models were developed: a normal diet group (ND), a high-fat diet group (HFD), a normal diet plus fecal microbiota transplantation group (ND-FMT), and a high-fat diet with fecal microbiota transplantation group (HFD-FMT). The objective was to examine the effects of high-fat diet-induced gut microbiota dysbiosis on female fertility. The results revealed that the HFD group exhibited significantly lower rates of oocyte maturation, embryo development rate, litter size, and oocyte quality compared with the ND group. Evidence from body mass measurements, blood glucose detection, and 16S rDNA sequencing confirmed the successful establishment of a gut microbiota dysbiosis model in mice following fecal mi- crobiota transplantation, with the HFD-FMT group showing a marked reduction in the relative abundance of Bacteroidetes and a concomitant increase in that of Firmicutes when compared with the ND-FMT group. Most critically, the maturation rate of oocytes, embryo development rate, and oocyte quality in the HFD-FMT group were substantially inferior to those in the ND-FMT group. Collectively, these data suggest that the imbalance of gut microbiota caused by a high-fat diet can adversely affect female reproductive capacity.
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