The Impacts of Erianin on the Proliferation, Apoptosis, and Epithelial-Mesenchymal Transition of Thyroid Cancer TPC-1 Cells by Regulating the Fas/FasL Signaling Pathway
GAO Dan1, ZHAO Shaoqing2, GAO Xiaowei3*
This study aims to research the effect of Erianin on the proliferation, apoptosis, and EMT (epi thelial-mesenchymal transition) of thyroid cancer TPC-1 cells by regulating the Fas/FasL signaling pathway. Hu man thyroid cancer cell line TPC-1 was cultured in vitro, and its subcutaneous transplant tumor and lung metastasis model was established in nude mice. The mice were assigned into a control group, a Erianin group, a Erianin+si-NC group, and a Erianin+si-Fas group randomly. After grouping intervention with Erianin, Fas siRNA negative control and Fas siRNA, the CCK-8 method and plate clone formation experiment were used to detect the proliferation abil ity of TPC-1 cells. Flow cytometry and Transwell assays were used to detect the apoptosis and metastasis abilities of TPC-1 cells. Immunohistochemistry was used to detect the apoptosis and EMT related proteins of TPC-1 cells in each group. The volume of subcutaneous transplanted tumor models in nude mice and the number of lung nodules in nude mice with lung metastasis models in each group were measured. Immunohistochemistry was used to detect the EMT related proteins in subcutaneous transplanted tumors of TPC-1 nude mice in each group. Western blot was used to detect the Fas/FasL signaling pathway proteins in TPC-1 cells in various groups, subcutaneous transplanted tumors, and lung nodule tissues of nude mice. The results showed that compared with the control group, the Erianin group and Erianin+si-NC group showed a decrease in cell D450, clone number, migration number, invasion number, tumor volume, lung nodule number, and N-cadherin positivity rate (P<0.05), and an increase in apoptosis rate, E-cadherin, caspase3, Bax positivity rate, the Fas and FasL (P<0.05). Compared with the Erianin group, the Erianin+si-Fas group showed an increase in cell D450, clone number, migration number, invasion number, tumor volume, lung nodule num ber, and N-cadherin positivity rate (P<0.05), and a decrease in apoptosis rate, E-cadherin, caspase3, Bax positivity rate, the Fas and FasL (P<0.05); there were no prominent changes in various indicators in the Erianin+si-NC group (P>0.05). This indicates that Erianin can induce apoptosis of thyroid cancer cells and inhibit their proliferation and EMT, possibly by activating the Fas/FasL signaling pathway.
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