Effect of Liz1 Gene Deletion on Mitotic Kinetics in Fission Yeast
RUAN Yuhan1, PAN Feng2, LIN Yintang2, REN Xingrui2, HOU Yiling2, DING Xiang1*
The Liz1 gene encodes the plasma membrane pantothenate transmembrane transporter protein Liz1, which is localized to the Golgi apparatus and plasma membrane. Liz1 gene deletion leaded to abnormalities of microtubule cytoskeleton, cytoplasmic division, and cell cycle during the mitosis in Schizosaccharomyces pombe. However, the underlying mechanism of the impact of Liz1 gene deletion on cell mitosis has not been fully revealed. This study used live cell imaging technology to observe the fluorescence labeled Liz1Δ strain to explore the effects of Liz1 gene deletion on the growth rate, sporulation quantity, and dynamics of microtubules, actin, and histones during mitosis of the Liz1Δ strain under standard temperature and high temperature stress. The results showed that at standard temperatures, Liz1 gene deletion resulted in slow cell growth with a 15% delay in spindle elongation time (P<0.01), a 22% delay in chromosome segregation to cell separation time (P<0.01) and a 12% delay in actin ring formation time (P<0.05). Under high-temperature stress, the slow growth of Liz1Δ strains was exacerbated, and the total time for actin ring formation to contraction was delayed by 14% (P<0.05). The data indicate that the deletion of the Liz1 gene leads to delayed cell growth, and its key mechanism is to delay the elongation of the spin dle in the anaphase of mitosis and the formation of actin ring, which provides a scientific basis for further clarifying the function of Liz1 protein in cell mitosis.
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