YE Danli1, CHEN Xiaolong1, LIN Huiwen1, LIN Binwei2, QI Pengliang1*
YE Danli1, CHEN Xiaolong1, LIN Huiwen1, LIN Binwei2, QI Pengliang1*
This article explores the effect of ciprofol on migration and invasion of colorectal cancer cells by regulating Hedgehog/Gli1 signaling pathway. SW480 cells were assigned into control group, low concentration cip rofol group (50 μmol/L ciprofol), medium concentration ciprofol group (100 μmol/L ciprofol), high concentration ciprofol group (150 μmol/L ciprofol), and high concentration ciprofol+SAG group (150 μmol/L ciprofol+200 nmol/L Hedgehog pathway activator SAG). CCK-8 method was used to detect the proliferation ability of cells. Plate cloning experi ment was used to detect the cloning ability of cells. Scratch healing experiment was used to detect the migration ability of cells. Transwell experiment was used to detect the invasion ability of cells. Flow cytometry was used to observe the apoptosis of cells in each group. Moreover, Western blot method was used to detect the expression levels of Bcl-2, Bax, cleaved-Caspase-3, SMO, Gli1, and SHH proteins of cells in each group. The results showed that compared with the control group, the low, medium, and high concentration ciprofol groups showed a decrease in cell D450 value, colony formation rate, cell scratch healing rate, invasive cell number, Bcl-2, SMO, Gli1, SHH, MMP-2 and MMP-9 protein expression levels, and an increase in apoptosis rate, Bax, cleaved-Caspase-3, and E cadherin protein expression levels (P<0.05). And with the increase of ciprofol concentration, the cell D450 value, colony formation rate, cell scratch healing rate, invasive cell number, Bcl-2, SMO, Gli1, SHH, MMP-2 and MMP 9 protein expression levels gradually declined, while the cell apoptosis rate, Bax, cleaved-Caspase-3 and E-cadherin protein expression levels gradually raised (P<0.05). Compared with the high concentration ciprofol group, the high concentration ciprofol+SAG group showed an increase in cell D450 value, colony formation rate, cell scratch healing rate, invasive cell number, Bcl-2, SMO, Gli1, SHH, MMP-2 and MMP-9 protein expression levels, and a decrease in apoptosis rate, Bax, cleaved-Caspase-3, and E-cadherin protein expression levels (P<0.05). In conclu sion, ciprofol may inhibit migration and invasion of colorectal cancer cells by suppressing Hedgehog/Gli1 signal ing pathway.
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