Impacts of circ-PHC3 on the Proliferation and Apoptosis of Cervical Cancer Cells by Targeting miR-628-5p
WANG Sha*, LIU Peng, FU Miao, TIAN Wen, YIN Xiaomei, WANG Shuqin, LIU Hao
This article aims to investigate the effect of miR-628-5p targeted by circ-PHC3 (circular RNA PHC3) on the proliferation and apoptosis of cervical cancer cells. Cervical cancer cells SiHa were grouped into interference control group, interference PHC3 group, overexpression control group, overexpression miR-628-5p group, interference PHC3+inhibition control group, interference PHC3+inhibition miR-628-5p group, and trans fected with corresponding plasmids. qRT-PCR was performed to measure the circ-PHC3 and miR-628-5p. CCK 8 method was used to measure cell proliferation. Flow cytometry was performed to detect cell apoptosis. Transwell method was performed to measure cell migration. Western blot was used to detect the Bcl-2 (B-cell lymphoma-2) and BAX (Bcl-2 associated X protein). Dual luciferase reporter gene was performed to explore the targeting rela tionship between circ-PHC3 and miR-628-5p. Compared with normal human cervical epithelial cells HUCEC, the circ-PHC3 increased and the miR-628-5p decreased in cervical cancer cell lines HeLa, C33A, SiHa, and ms751 (P<0.05). Compared with the interference control group, the miR-628-5p in SiHa cells in the interference PHC3 group increased, the proliferation and migration abilities, and Bcl-2 expression level decreased, and the apoptosis rate and BAX expression level increased (P<0.05). Compared with the overexpression control group, the proliferation and migration abilities, and Bcl-2 expression level in the miR-628-5p overexpression group decreased, and the apopto sis rate and BAX expression level increased (P<0.05). Compared with the interference PHC3+inhibition control group, the miR-628-5p in SiHa cells in the interference PHC3+inhibition miR-628-5p group decreased, the proliferation and migration abilities, and Bcl-2 expression level increased, and the apoptosis rate and BAX expression level decreased (P<0.05). Gene analysis reported that there was a targeted relationship between circ-PHC3 and miR-628-5p (P<0.05). Circ-PHC3 promotes proliferation of cervical cancer cells and inhibits apoptosis of cervical cancer cells by competi tively binding and downregulating miR-628-5p.
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