Research Advances and Multidimensional Applications of Gastric Organoids: from Basic Construction to Preclinical Studies

MU Xinru^1,2, XUE Fan^1,2, PANG Minjiao^1,2, TONG Qiyue^1,2, ZHANG Jianing^1,2, MIAO Zhifeng^1,2*

(¹Department of Surgical Oncology and General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China; ²Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, the First Affiliated Hospital of China Medical University, Shenyang 110001, China)

Abstract:

Organoids are three-dimensional culture systems derived from stem cells that have shown great promise in disease modeling, drug screening, and personalized medicine. This review first outlines the basic construction process of gastric organoids and summarizes recent advances in matrix materials, supportive culture systems, and quality control measures. It also compares organoid models with traditional systems, highlighting their advantages and limitations. In the application section, the role of normal gastric organoids in developmental biology and disease modeling is discussed, along with the potential and challenges of gastric cancer organoids in studying drug resistance and guiding individualized therapy.

CSTR: 32200.14.cjcb.2025.09.0017