Constructing Biomimetic Tissue and Organ Models Using Organoid-on-a-Chip
SONG Ke1,2,3, WU Xinghan1,2, LIU Yaling1,2*
Biomimetic tissue and organ models are engineered to replicate the biological, physical, and chemical properties of natural tissues and organs. These model systems serve as experimental platforms to provide critical insights into tissue development, disease progression, and pharmacological response. OoC (organoid-on-achip) technology, which integrates organoids with organ-on-a-chip platforms, has shown considerable promise in the construction of biomimetic tissues and organs in recent years. By combining chip fabrication, microfluidics, and detection technologies, OoC systems allow for the precise simulation of the dynamic features of human tissues and organs, providing essential tools for disease research, drug screening, and regenerative medicine. Despite advancements in the application of OoC technology in single-tissue and organ models, challenges remain in developing more complex multi-organ interaction models and constructing tumor models. This review examines strategies for constructing biomimetic tissue and organ models, discusses the application of OoC technology in single-tissue and organ models, such as the liver, heart, and epithelium, as well as multi-organ interaction models, including twotissue and three-tissue OoC. It also discusses the advantages and limitations of OoC in simulating organ-specific cellular behaviors and physiological functions. Additionally, the application of OoC technology in tumor models is detailed, emphasizing its potential in anti-tumor drug screening and personalized medicine. The review concludes with an outlook on current challenges and future development directions of OoC models, aiming to promote OoC model translational potential and provide new insights for the construction of biomimetic tissue and organ models.
CN
EN