Applications and Optimization Strategies of Novel Matrix Materials in Organoid Culture
LIU Yating, YI Xiao*
Organoids, as three-dimensional in vitro models of miniature organs, rely heavily on microenvironmental regulation mediated by matrix materials for their stable construction and functional maturation. Such regulation is critical for biological processes including organoid differentiation, proliferation, adhesion, morphogenesis, and phenotypic expression. Conventional matrix materials (e.g., Matrigel) are unable to meet the demands of standardized research and clinical translation due to their complex composition, significant batch-to-batch variability, and risks of animal-derived contamination, driving the development of novel matrix materials as a research priority. This review focuses on emerging matrix materials, proposing optimization strategies from four perspectives: chemical composition, structural design, physical properties, and integration of biological signals, while analyzing their application progress in organoid culture. Future research should further refine matrix materials to reconstruct the extracellular matrix of source tissues, accurately mimic tissue-specific microenvironments, and guide directional differentiation to promote the formation of internal spatial structures. These advancements will significantly enhance the maturity and physiological functionality of organoids, accelerating their clinical translation.
CN
EN