Impacts of CircACAP2 on the Proliferation, Migration, and Invasion of Glioma Cells by Regulating miR-139-5p/HOXA9 Signaling Axis
WU Jie, YANG Yuhao, QIU Zhaoyou*
This study aims to investigate the effects of CircACAP2 on the proliferation, migration, and invasion of glioma cells by regulating the miR-139-5p (microRNA-139-5p)/HOXA9 (homeobox gene A9) signaling axis. From December 2020 to December 2024, 150 cases of glioma tissue of patients and 150 cases of normal brain tissue from patients who underwent intracranial decompression surgery due to trauma and other reasons
were collected in the hospital. The glioma cell line SW1088 was used as the research object and classified into CK group, silencing-NC group, silencing-CircACAP2 group, miR-NC group, miR-139-5p mimic group, silencing-CircACAP2+inhibitor NC group, and silencing-CircACAP2+miR-139-5p inhibitor group. qRT-PCR was used to detect the CircACAP2, miR-139-5p, and HOXA9 mRNA in tissues and SW1088 cells. CCK-8 method, scratch assay, Transwell method, and Western blot method were used to detect cell proliferation, migration, invasion, and related proteins, respectively. The dual luciferase assay was used to verify the targeting relationship between miR-139-5p and CircACAP2, HOXA9. The results showed that the expression levels of CircACAP2, HOXA9 mRNA, and HOXA9 protein were increased, while the expression level of miR-139-5p was decreased in glioma tissues compared with normal brain tissues (P<0.05). Compared with the CK group and silencing-NC group, the CircACAP2 group showed decreased CircACAP2, HOXA9, cell survival rate, scratch healing rate, number of invasive cells, the expression of Ki-67, and Vimentin proteins, and increased miR-139-5p (P<0.05). Compared with the CK group and miR-NC group, the miR-139-5p mimic group showed decreased HOXA9, cell survival rate, scratch healing rate, number of invasive cells, the expression of Ki-67, and Vimentin proteins, and increased miR-139-5p (P<0.05). Compared with the silencing-CircACAP2+miR-139-5p inhibitor group and silencing-CircACAP2+inhibitor NC group, the silencing-CircACAP2+miR-139-5p inhibitor group showed reduced miR-139-5p, increased HOXA9 mRNA, cell survival rate, scratch healing rate, number of invasive cells, the expression of Ki-67, Vimentin, and HOXA9 proteins (P<0.05). CircACAP2 and HOXA9 had targeted relationships with miR-139-5p, respectively. The results indicated that silencing CircACAP2 could up-regulate the expression of miR-139-5p, which in turn inhibited the expression of HOXA9, thereby suppressing the proliferation, migration, and invasion of glioma cells.
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