LncRNA PART1 Alleviates High Glucose-Induced Renal Tubular Epithelial Cell Injury by Targeting miR-301b-3p

This study aims to explore the effect of lncRNA PART1 on the injury of human renal tubular epithelial cells HK-2 induced by high glucose and its possible mechanism. High glucose-induced HK-2 cells were used to establish a cell injury model, and randomly grouped into groups: Con group, HG group, HG+pcDNA group, HG+pcDNA-PART1 group, HG+anti-miR-NC group, HG+anti-miR-301b-3p group, HG+pcDNA-PART1+miR-NC group, HG+pcDNA-PART1+miR-301b-3p group. qRT-PCR was used to detect the expression of lncRNA PART1 and miR-301b-3p. ELISA method was used to detect the levels of IL-6 and TNF-α. Flow cytometry was used to detect the rate of apoptosis. The dual luciferase reporter experiment was used to verify the targeting relationship between lncRNA PART1 and miR-301b-3p. Western blot was used to detect the protein expression of cleaved-caspase3 and cleaved-caspase9. Compared with the Con group, the expression of lncRNA PART1 in the HG group was decreased (P<0.05), while the expression of miR-301b-3p, the levels of IL-6 and TNF-α, cell apoptosis rate, and cleaved-caspase3 and cleaved-caspase9 protein levels were increased (P<0.05). Compared with the HG+pcDNA group, IL-6, TNF-α levels, the apoptosis rate and the protein levels of cleaved-caspase3 and cleaved-caspase9 were decreased in the HG+pcDNA-PART1 group (P<0.05). LncRNA PART1 could target miR-301b-3p. Compared with the HG+anti-miR-NC group, the levels of IL-6, TNF-α, apoptosis rate and cleaved-caspase3 and cleaved-caspase9 protein levels were decreased in the HG+anti-miR-301b-3p group (P<0.05). Compared with the HG+pcDNAPART1+ miR-NC group, the levels of IL-6, TNF-α, the rate of apoptosis and the protein levels of cleaved-caspase3 and cleaved-caspase9 were increased in the HG+pcDNA-PART1+miR-301b-3p group (P<0.05). LncRNA PART1 can inhibit the inflammatory response and apoptosis by down-regulating the expression of miR-301b-3p, thereby reducing the damage of renal tubular epithelial cells induced by high glucose.

CSTR: 32200.14.cjcb.2025.07.0013