Effect of Low-Power WPT Electromagnetic Environment on NeuronalExcitability in Hippocampal CA1 Region

ZHAO Jun^1,2,3*, WANG Xiaoxuan^1,2,3, ZHANG Bingqian^1,2,3, MA Jing^1,2,3

(¹Hebei University of Technology Life Health and Engineering College, Tianjin 300130, China; ²Key Laboratoryof Bioelectromagnetic and Neural Engineering of Hebei Province, Hebei University of Technology, Tianjin 300130, China;³State Key Laboratory of Reliability and Intelligence of Electrical Equipment, Hebei University of Technology, Tianjin 300130, China)

Abstract:

The aim of this study was to investigate the effects of low-power WPT(wireless power transmission) system on the excitability of hippocampal CA1 neurons in mice. Mice were divided into control group andradiation group (2-week group, 4-week group, 6-week group). Morris water maze test, fiber photometry test, HEstaining test, patch clamp test were used to observe the changes of working memory ability, Ca2+ signal, the number of hippocampal pyramidal cells, action potential, IA(instantaneous outward K+ channel current) and IK(delayedrectifier K+ channel current) in mice. The results of Morris water maze test showed that low-power WPT electromagnetic environment did not affect the working memory ability of mice. Fiber photometry test and HE stainingtest showed that low-power WPT electromagnetic environment may promote the discharge activity of hippocampalCA1 neuron clusters, resulting in an increase in fluorescence signal. This suggests that the regulation of Ca2+ concentration by electromagnetic environment might increase the number of discharge activities of hippocampal CA1neurons and enhance the excitability of hippocampal CA1 neurons. With the increase of radiation time, the peakvalue of fluorescence signal gradually decreased, indicating that hippocampal pyramidal cells of mice adapted tolow-power WPT electromagnetic environment. Low-power WPT electromagnetic environment increases the restingmembrane potential of hippocampal CA1 neurons in mice, shortens the half-wave width of action potential, reducesthe action potential threshold, accelerates the frequency of action potential release, promotes the release of actionpotential, and improves the excitability of hippocampal CA1 neurons. Low-power WPT electromagnetic environment inhibits the activation process of transient outward potassium channels on the cell membrane, delays the activation characteristics of rectifier potassium channels to the depolarization direction, reduces the outflow of K+ in thecell, and then enhances the excitability of hippocampal CA1 neurons. Low-power WPT electromagnetic environment promotes the discharge activity of hippocampal vertebral cells, inhibits the activation process of IA and IK, andthe activation curve of IA channel shifts to the depolarization direction, enhancing the excitability of neurons.