Effect of Xanthones from Gentianopsispaludosa on Mesenchymal Transformation of NCM460 Cells
LIU Na, LIU Yuemin, BO Shuangqin, CHEN Hui, ZHANG Shangzhi, JING Ming*
This study investigated the effects of Xanthones from Gentianopsispaludosa on human NCM460 (normal colon mucosa cell) interstitial conversion. NCM460 cells were induced with 2.5 μg/mL LPS (lipopolysaccharide) for 14 d to establish interstitial transformation model, and were treated with GPX and PFD respectively. The expression of E-cadherin and Vimentin protein and mRNA were detected by IF (immunofluorescence), WB (Western blot) and RT-qPCR (real-time fluorescence quantitative transcription polymerase chain reaction), respectively. The results showed that compared with normal control group, the epithelial cell marker E-cadherin protein and mRNA in model control group were significantly down-regulated (P<0.01), interstitial cell marker Vimentin protein and mRNA were significantly up-regulated (P<0.01). Compared with the model group, the fluorescence intensity of E-cadherin was positively correlated with the dose of GPX, while the fluorescence intensity of Vimentin was decreased in a dose-dependent manner. The expression of E-cadherin protein and mRNA was up-regulated and that of Vimentin protein and mRNA was down-regulated in GPX medium and high dose groups (P<0.01), most obvious in high dose. The study showed that Xanthones from Gentianopsispaludosa could effectively inhibit colon EMT (epithelial mesenchymal transformation), which provided theoretical basis for further research and development.
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