Effects of hsa_circ_0000745 on Proliferation, Invasion and Migration of Hepatocellular Carcinoma Cells and Its Mechanism

This study was to investigate tumor-promoting effect of circular RNA hsa_circ_0000745 on hepatocellular carcinoma cells and its related mechanism. Firstly, quantitative Real-time PCR (qRT-PCR) was used to detect the expression of circular RNA hsa_circ_000745 in hepatocellular carcinoma cells. Secondly, hsa_ circ_000745 in HepG2 cells was knocked down by siRNA interference technology, and then CCK-8 and plate cloning experiments were used to detect the proliferation ability of cells. Transwell test and scratch healing test were used to detect cell invasion and migration ability. Western blot was used to detect the related proteins of proliferation, invasion and migration. The results showed that after interfering with hsa_circ_0000745, the proliferation and clonogenic ability of hepatocellular carcinoma cells were decreased (P<0.05), and the expression of cyclin D1 was downregulated (P<0.001). The migration and invasion abilities of cells were decreased (P<0.05). The expression of epithelial marker E-Cadherin was up-regulated (P<0.05), and the expressions of interstitial markers N-Cadherin and Vimentin were down-regulated (P<0.05). The results showed that knockdown of circular RNA hsa_circ_0000745 significantly inhibited the proliferation, migration, invasion and EMT (epithelial-mesenchymal transition) of hepatocellular carcinoma cells, which provided a new potential target and perspective for the diagnosis and treatment of hepatocellular carcinoma.