SIK1, As a New Target Gene of miR-93, Inhibits the Proliferation, Invasion and Migration of Prostate Cancer Cells

YANG Xu¹, XIE Hui^1,2, LIN Ping¹, YU Xiaoguang¹*

(¹Department of Biochemistry and Molecular Biology, Basic Medicine College, Harbin Medical University, Harbin 150081, China; ²Biotechnology Experimental Center, Basic Medicine College, Harbin Medical University, Harbin 150081, China)

Abstract:

This paper investigated the inhibitory effect of SIK1, as a new target gene of miR-93, on the proliferation, invasion and migration of prostate cancer cells. After the expression of SIK1 in prostate cancer cells was up-regulated by recombinant plasmid pcDNA3.1-SIK1, CCK8 and clone formation assay were used to detect cell proliferation; cell invasion and migration was detected by cell scratches and Transwell assay; the protein expression levels of E-cadherin and Vimentin were detected by Western blot. Luciferase reporting experiment and Western blot verified the targeted regulation of SIK1 by miR-93. The results showed that up-regulated SIK1 could inhibit the proliferation, invasion and migration of prostate cancer cells, as well as increase E-cadherin protein expression and decrease Vimentin protein expression; miR-93 could negatively regulate SIK1. In conclusion, SIK1 can act as a new target gene of miR-93 to inhibit the proliferation, invasion and migration of prostate cancer cells.