Effects of Asiat on Oxidative Stress, Fos Protein and Ki67 Protein in Rats with Cerebral Infarction

To explore the effects of Asiat on oxidative stress and the protein expression of Fos and Ki67 in rats with CI (cerebral infarction), sixty rats were randomly assigned into sham group (sham operation group), CI group (CI model group) and CI operation plus Asiat group (Asiat treatment group). Then, the neurobehavioral, hydrocephalus, and CI volume of rats in each group were evaluated. Cytokine concentration was detected by ELISA. Morphology of brain tissues was observed by HE staining. Additionally, neuronal cell apoptosis was detected by TUNEL staining. Fos protein and Ki67 protein expression were detected by Western blot. Results showed that the concentrations of NO and MDA were significantly higher, but the activity of SOD was reduced in the CI group compared with those in the sham group (P<0.05). Compared with rats in the CI group, the concentrations of NO and MDA decreased significantly, and the activity of SOD increased significantly in the Asiat group (P<0.05). In CI group, the amount of hydrocephalus in brain tissues, the volume of CI and the apoptosis of neuronal cells increased significantly compared with sham group. In addition, the cytoplasm was stained unevenly; the nucleus was stained deeper; and the number of neurons was lower in CI group than those in sham group. On the contrary, the hydrocephalus, CI and neuronal cells apoptosis were obviously attenuated, and the morphology of brain tissues was significantly improved in the Asiat group. Compared with the sham group, the expression of Fos protein increased, while the expression of Ki67 protein decreased in the CI group (P<0.05). However, Asiat treatment recovered Fos and Ki67 expressions (P<0.05). In conclusion, this study showed that Asiat could inhibit the oxidative stress response of rats with CI, and the expression of Fos protein, and promote the expression of Ki67 protein, thereby exhibiting its protective effect on brain tissues.