LncRNA OSER1-AS1 Affects the Proliferation, Migration and Invasion of Cervical Cancer Cells by Regulating miR-433-3p

 This study is aimed to explore the effect of lncRNA OSER1-AS1 on the proliferation, migration and invasion of cervical cancer cells and its regulation on miR-433-3p. Western blot and qRT-PCR were used to detect the expression levels of OSER1-AS1 and miR-433-3p in cervical cancer cells, finding that the expression level of OSER1-AS1 in cervical cancer cells was significantly increased (P<0.05), while the expression level of miR433-3p was significantly reduced (P<0.05). si-NC, si-OSER1-AS1, miR-NC, miR-433-3p, si-OSER1-AS1+antimiR-NC, and si-OSER1-AS1+anti-miR-433-3p were transfected into SiHa cells. Furthermore, CCK-8 method and plate cloning experiment were used to detect cell proliferation ability, finding that transfection of si-OSER1-AS1 or miR-433-3p mimics significantly reduced cell viability (P<0.05), and the number of clone formation, migration and invasion cells were significantly reduced (P<0.05). However, when transfected with si-OSER1-AS1+anti-miR433-3p, cell viability was significantly increased (P<0.05), and the number of clone formation, migration and invasion cells were significantly increased (P<0.05). Finally, the dual luciferase reporter experiment was used to detect the targeting relationship of OSER1-AS1 and miR-433-3p, finding that OSER1-AS1 could competitively bind to miR-433-3p. The results indicated that OSER1-AS1 could negatively regulate the expression of miR-433-3p and mediate cell proliferation, migration and invasion. It was suggested that interference with OSER1-AS1 expression could inhibit the proliferation, migration and invasion of cervical cancer cells by up-regulating the expression of miR-433-3p.