Preparation of Anti-Human TTC36 Monoclonal Antibody and Its Application in Immunohistochemistry Based on Polypeptides Antigen

This study aimed to investigate the function of anti-TTC36 monoclonal antibodies, especially its application in immunohistochemistry. Four TTC36 polypeptides coupled with KLH were used as antigens to immunize Balb/c mice. The corresponding monoclonal antibodies were screened and prepared by hybridoma method. ELISA and Western blot were used to identify and analyze the subtype, titer and specificity of monoclonal antibodies. The application was investigated with immunohistochemical techniques. Results showed that five monoclonal antibodies recognizing human TTC36 protein were obtained, which could specifically bind to TTC36 protein expressed by prokaryotic cells and eukaryotic cells. These monoclonal antibodies could recognize TTC36 protein in human liver and kidney tissue, which also indicated that TTC36 expression decreased in liver and kidney cancer compared with normal tissue. In conclusion, monoclonal antibodies that bind to human TTC36 have been successfully prepared and can apply in immunohistochemistry, which provides the basis for the clinical diagnosis and drug research of related diseases.