Effects of Ethanol Extract of Diaphragma Juglandis Fructus on the Proliferation, Apoptosis and Migration of Colon Cancer HCT116 Cell

The present study aims to explore the effects of EEDJF (ethanol extract of diaphragma juglandis fructus) on the proliferation, apoptosis and migration of human colon cancer cell HCT116. The bioactive ingredients of diaphragma juglandis fructus were extracted with 75% ethanol. MTT assay was applied to determine the effect of EEDJF on HCT116 cell proliferation. AO/EB double staining, TUNEL assay and Western blot were used to detect cell apoptosis. Scratch wound-healing assay was performed to determine the cell migration ability. Establishment of 3D cell culture model of HCT116 cells was applied to evaluate the anti-proliferation effect of EEDJF in vitro. The results showed that EEDJF increased the expression of pro-apoptotic factor Bax and the cleavage of PARP, meanwhile, decreased the expression of anti-apoptotic factor Bcl2. Additionally, the results of Scratch wound-healing assay and 3D cell culture showed that EEDJF inhibited the migration of HCT116 cells and the proliferation of 3D-cultured HCT116 cells. Thus, the present study demonstrats that EEDJF inhibits the proliferation and migration of HCT116 cells, and induces the apoptosis of HCT116 cell by up-regulating the expression of cleaved PARP and Bax, and decreasing Bcl2. Moreover, EEDJF inhibits the proliferation of 3D-cultured HCT116 cells. Therefore, this study suggests that there is no significant difference between in vitro and in vivo in the sensitivity of HCT116 cells to EEDJF.