Comparison of Secreted Proteins between Human Skin Fibroblasts and Umbilical Cord Mesenchymal Stem Cells

DUAN Jing¹, WANG Yan¹, WANG Wei¹, LU Xianjie¹, LIU Yanming¹, WANG Xiaobing², PANG Baojun³, HAN Fabin^1,4*

(¹The Institute for Tissue Engineering and Regenerative Medicine, the Liaocheng People’s Hospital/Liaocheng University, Liaocheng 252000, China; ²Gynecology and Obstetrics, the Liaocheng People’s Hospital/Liaocheng University, Liaocheng 252000, China; ³Clinical laboratory, the Liaocheng People’s Hospital, Liaocheng 252000, China; ⁴Institute of Translational Medicine, the Second Hospital of Shandong University, Jinan 250013, China)

Abstract:

To elucidate the probable mechanism of hUC-MSCs (umbilical cord mesenchymal stem cells) secretory proteins in the treatment of neurodegenerative diseases, this study investigated the differences in secretory proteins between NCF (human skin fibroblasts) and hUC-MSCs. Based on LC-MS/MS (tandem mass spectrometry), the supernatant samples of hUC-MSCs and NCF cultured in serum-free medium for 48 hours were collected for TMT (tandem mass tags for relative and absolute quantitation) to detect peptides after protein digestion. Isotope labeling was performed, and then the difference in expression of secretory proteins was accurately identified and quantified. The secretory proteins of NCF and hUC-MSCs were established by bioinformatics analysis, and functional analysis of these secretory proteins were performed by GO (gene ontology). Proteins at the central position were analysed by Real-time PCR, such as CCL2, COL4A1, TGFβ1, SERPINE1 and SEMA7A. More than 1 000 consensus proteins were identified and the corresponding functions were searched by Uniprot. And the paper confirmed that the secretion of CCL2 and COL4A1 in hUC-MSCs were more than the secretion in NCF. The paper find that these two molecules play an important role in the elimination of inflammation, and the inflammatory response plays an important role in the occurrence and development of degenerative diseases such as Parkinson’s disease. By analyzing the proteins secreted in NCF and hUC-MSCs culture medium, this study lays the foundation for elucidating the differences in molecular mechanisms between mesenchymal stem cells and skin fibroblasts.