The Biological Behavior Effect of Overexpression of lncRNA CASC2a in Human Colon Cancer Cell SW480

MA Yu¹, ZHANG Jie¹, WANG Lanyu¹, LI Zhenxing¹, TIAN Wei², ZHANG Guozhi¹, CHEN Jianli¹*

(¹Affiliated Hospital of North China University of Science and Technology, Department of General Surgery, Tangshan 063000, China; ²Medical Experimental Research Center, North China University of Science and Technology, Tangshan 063000, China)

Abstract:

This study was to investigate the effect of overexpression of lncRNA CASC2a in human colon cancer cell SW480 on cell proliferation, migration, invasion and apoptosis. The human colon cancer cell line SW480 was selected to construct the lncRNA CASC2a overexpression plasmid model, and the content of lncRNA CASC2a in the cells was detected by qRT-PCR. Cell proliferation, migration, invasion and apoptosis were compared by CCK-8, scratch test, Transwell, Caspase-3 activity test and TUNEL test. The expression of apoptotic antibodies Cleaved Caspase-3, Caspase-3, BCL-2 and Bax was detected by Western blot. The results showed that after overexpression of lncRNA CASC2a, the proliferation, migration, and invasion capacity of SW480 human colon cancer cells all reduced and the apoptosis ability enhanced. The expression of Cleaved Caspase-3, Caspase-3, and Bax protein increased, and the expression of BCL-2 decreased. The study concludes that overexpression of lncRNA CASC2a can inhibit the proliferation, migration and invasion of human colon cancer cell SW480, and induce apoptosis.