Regulation Mechanism of SNRPA1 on the Tumorigenicity of Hepatocellular Carcinoma

SNRPA1 (small nuclear ribonucleoprotein A) is involved in assembly of mRNA-processed splicesome and associated with tumorigenesis of many tumors. However, it’s function in the molecular mechanism for tumorigenicity of HCC (hepatocellular carcinoma) remains unclear. In this study, gene chip technology was used to explore the expression dynamics of key genes in HCC cell signaling pathway after SNRPA1 knockdown, and its molecular mechanism for regulating the development of HCC in nude mice. The results of optical in vivo imaging showed that the growth of SNRPA1 deleted HCC in nude mice was significantly inhibited compared with the control group. Gene chip analysis showed that knockdown of SNRPA1 led to 462 genes downregulation and 262 genes up-regulation. qRT-PCR analysis showed that the expression of FSTL1, FGF2, JAK2, WNT5A and PPM1A were all decreased, while Western blot analysis further confirmed that the expression of FSTL1, JAK2, WNT5A were down-regulated. The above results show that SNPRA1, as a pro-oncogene, may be involved in the tumorigenicity and development of hepatocellular carcinoma through regulating multiple genes and signal pathways.