Study on Effects of Soybean Isoflavone on Oleic Acid-Induced Liver Injury in Rats

GAO Shuang^1#, TAN Jinlong^1#, LUO Qihui^1,2, HUANG Chao¹, LIU Wentao^1,2, CHEN Zhengli^1,2*

(¹Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; ²Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China)

Abstract:

To investigate the effect of SIF (soy isoflavone ) on oleic acid-induced liver injury in rats, the rat model of liver injury was established by injecting oleic acid into tail vein of SD (sprague dawley) rats at 6 weeks old. Blood biochemical examination, hematoxylin eosin staining, immunohistochemistry and real-time fluorescence quantitative PCR were used to explore the liver injury caused by oleic acid and the intervention of soybean isoflavone, the content of ALT and AST in blood, the morphology of liver tissue, the positive distribution of TNF-α and IL-6 protein in liver tissue and the transcription level of their mRNA were detected. The results showed that compared with the basal control group, the content of ALT and AST in the blood of the vehicle control group was increased significantly, and the liver cells showed more serious diffuse edema, vacuolation, and a small amount of necrosis, after SIF intervention, ALT and AST contents in blood of high, middle and low dose groups were significantly reduced, and athology of liver cells were significantly improved. The results of immunohistochemistry showed that compared with the basal control group, the liver cells of the vehicle control group showed high expression of TNF-α and IL-6, after the intervention of SIF, the expression of TNF-α and IL-6 in the high, middle and low dose groups decreased significantly. The results of real-time fluorescence quantitative PCR showed that the mRNA transcription level of TNF-α and IL-6 was basically consistent with the protein expression of immunohistochemistry. The above results showed that after continuous gavage for a period of time, different doses of soy isoflavone can reduced the expression of TNF-α and IL-6 in liver tissue induced by oleic acid, and improved oleic acid induced liver injury, Further exploration of the exact mechanism of action of soybean isoflavones on oleic acid liver injury is beneficial to the clinical development and application of SIF.