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The Construction of Transcription Activator-like Effector Nucleases (TALENs) and Its Application in the Genome Fixed-point Modification
Zhou Jinwei1, Wang Linghui2, Shen Yijun1, Yu Shumin1, Cao Suizhong1*
1College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625000, China; 2College of Life Science, Sichuan University,Key Laboratory of Biological Resources and Ecological Environment , the Ministry of Education, Chengd
Abstract: Transcription activator effector nucleases (TALENs) is a novel DNA editing technology which can induce precise site-specific modification in genome. TALENs consist of two distinct domains， an engineered DNA-binding domain derived from the transcription activator-like effector (TALE) that provides the capability and specificity for binding target DNA sequence， and a non-specific restriction endonuclease Fok I domain fused to the C-terminal of TALE that confers the nuclease activity of TALENs. Here， we summarize the technological background and provide an overview of development and applications of TALENs in genome engineering.
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