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An Optimized Co-culture Model of Mouse Blastocysts and Melanoma Cells
Hong Zhang, Xiao-Yan Ding, Liao-Qiong Fang, Zhi-Biao Wang*, Hai Qiao
Chongqing Key laboratory of Ultrasound in Medicine and Engineering of Chongqing Science and Technology Commission, Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
Abstract: EGFP (Enhanced Green Fluorescent Protein) gene was transfected into B16 melanoma cells， and sorted out EGFP-B16 cells which expressed EGFP stably. EGFP mRNA was detected by RT-PCR (Reverse Transcription Polymerase Chain Reaction) and EGFP expression was analysed by flow cytometry (FC). EGFP-B16 cells were used to co-culture with mouse blastocysts derived from C57BL/6 mouse. B16 cells co-cultured with C57BL/6 mouse balstocysts were served as control groups. The co-culture of EGFP-B16 cells and blastocysts observed under laser confocal fluorescent microscope (LCFM) was better to display the interaction of embryos and tumor cells than control groups observed under inverted microscope.
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