Vol.30 No.3 (2008 June): 411-414

Methods for Vital Fluorescent Labeling of Guard Cells Vacuole

Xin-Qi Gao*, Qiu-Ping Ren¹, Zhuan-Bin Wang²

(State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China; ¹Agricultural College, Liaocheng University, Liaocheng 252029, China; ²College of Life Sciences, Qufu Normal University, Qufu 273165, China)

Abstract Vital labeling of vacuole is required for the study of vacuolar dynamics. Several methods for vacuole labeling of guard cells in vivo were introduced in this paper by using of acridine orange, Lysotracker Red DND-99, BCECF-AM, fluorescein diacetate and GFP fusion protein. We found that the procedures of vacuolar labeling by acridine orange and fluorescein diacetate are simple and the results are better. In addition, GFP fusion protein might be the best strategy for vital vacuolar labeling in the future.

Key words guard cell; vacuole; vital labeling

Received: December 7, 2007 Accepted: February 4, 2008
This work was supported by the National Natural Science Foundation of China (No.30770193)
*Corresponding author. Tel: 86-538-8246020, E-mail: gaoxq@sdau.edu.cn