Vol.30 No.3 (2008 June): 406-410

Studies of Cytoskeleton in Oocytes and Preimplantation Embryos by Confocal Laser Scanning Microscopy

Ying Xu^¡ø, Qing-Hua Zhang^1¡ø, Na Guan¹, Yan-Ning Xu¹, Xiao-Fei Yan¹, Lei Lei^1*

(Departpment of Anatomy and Histology, College of Medicine, Jiaxing University, Jiaxing 314001, China; ¹Department of Histology and Embryology, Harbin Medical University, Harbin 150081, China)

Abstract This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique. We observed the configuration, distribution and localization of β-tubulin, γ-tubulin, microfilaments and chromosomes in mouse oocytes and preimplantation embryos. We also described some key points and tricks of this technique, and discussed analysis method of the experiment results as well.

Key words confocal laser scanning microscopy; cytoskeleton; oocyte; preimplantation embryos

Received: November 6, 2007 Accepted: January 16, 2008
This work was supported by the Natural Sciences Foundation of China (No.30671025) and the Major Scientific Research Project of the Jiaxing Science and Technology Bureau (No.2007AZ2013)
¡øThese authors contributed equally to this work
*Corresponding author. Tel: 86-451-86674518, Fax: 86-451-87503325, E-mail: leil086@yahoo.com.cn