Vol.28 No.3（2006 June）：473-476

Genistein Inhibit Cathepsin K Expression Stimulated by IL-1a in Osteoclast-like Cells
Yun-Lin Wang^*, Xiao-Qing Liu, Fei Yang, Han-Hua Yao, Hong-Bing Wang, Qin Xia, Huan-Li Zhu
(Department of Gerontism, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China)

Abstract The osteoclast-like cells (OCLs) isolated from tissue of human giant cell tumor of bone, were stimulated by IL-1a in the presence of genistein. Then the expression of cathepsin K (CK) was determined by RT-PCR and Western blot in OCLs. Compared with control group, the expressions of CK were up-regulated after stimulated by IL-1a in other groups (P<0.01). Genistein down-regulated CK gene expression stimulated by IL-1a at the transcription level stimulated in dose-dependent manner (P<0.01) and its effect was abrogated partly after treated with the estrogen receptor antagonist ICI 182.780. Genistein inhibit CK expression stimulated by IL-1a through estrogen receptor in OCLs. We found IL-1a stimulated the expression of CK in OCLs.

Key words genistein; osteoclast-like cells; cathepsin K; IL-1a

Received: November 18, 2005 Accepted: February 27, 2006

This work was supported by the Natural Science Foundation of Hubei Province (No.2004ABA251)

^*Corresponding author. Tel: 86-27-83662044, Fax: 86-27-83662640, E-mail: wyl_wuhan@yahoo.com.cn