Vol.28 No.3（2006 June）：457-462

Effects of Protein Kinase C on Expression and Subcellular Distribution of Ankyrin and CD44 in Vascular Endothelial Cells
Yi-Wen Hao^1*, Da-Ye Cheng¹, Ya-Ming Li²
(¹Department of Transfusion, ² Department of Nuclear Medicine, First Affiliated Hospital,China Medical University, Shenyang 110001, China)

Abstract Membranous protein kinase C (PKC) activity after phorbol-myristate-acetate (PMA) treatment was determined by the incorporate quantity of [g-³²P]-ATP into exogenous substrate in human umbilical vein endothelial cells (HUVECs). Effects of PKC activity on subcellular distribution and protein expression of ankyrin and CD44 was analyzed respectively with indirect immunofluorescence and Western blot. We found that the trend of ankyrin and CD44 expression was identical with PKC activity change. PKC activation could result in CD44 aggregating on HUVECs membrane, and ankyrin also translocated and aggregated beneath CD44. Calphostin C could inhibit the above action of PKC. The results showed that PKC activation could up-regulate protein expression of ankyrin and CD44 by phosphorylation, and resulted in the coherent migration and colocalization of ankyrin and CD44 simultaneously.

Key words vascular endothelial cells; protein kinase C; CD44; ankyrin

Received: September 26, 2005 Accepted: February 28, 2006

^*Corresponding author. Tel: 86-24-83283038, Fax: 86-24-22703576, E-mail: HYW82666@163.com