|
Effect of Nano-selenium on Cellular Glutathione Peroxidase Activity in Chick Hepatocytes
Cai-Hong Hu*, Mei-Sheng Xia
(College of Animal Science; The Key Laboratory of Molecular Animal Nutrition, Ministry of Education,
ZheJiang University, Hangzhou 310029, China)
Abstract： A new selenium source, nano elemental selenium (Nano-Se) was used to study the effect on cellular glutathione peroxidase (cGPx) in chick hepatocytes, and sodium selenite (Na2SeO3) and seleno-1-methionine (Se-Met) were used as controls. Se was added to the chick hepatocytes at 0.01, 0.05, 0.1, 0.3, 0.5 and 1.0 µmol/L Se, respectively. The results showed that the cGPx activity was increased with elevation of Se concentrations in the range of 0.01-0.10 µmol/L for Na2SeO3 and 0.01-0.30 µmol/L for Se-Met or Nano-Se, respectively. The cGPx activity was decreased with elevation of Se concentrations in the range of 0.10-1.0 µmol/L for Na2SeO3 and 0.30-1.0 µmol/L for Se-Met, respectively. However, the cGPx activity still remained steady at a range of 0.30-1.0 µmol/L for Nano-Se. The results implicated that the ranking of width range of the most suitable Se concentration for nutrition curve of the three Se forms was: Nano-Se>Se-Met>Na2SeO3.
Key words： selenium; nano; cellular glutathione peroxidase; hepatocyte; chick
*Corresponding author. Tel: 86-571-86985607, Fax: 86-571-86961553, E-mail: chhu@zju.edu.cn
|