Vol.26 No.6（2004 December）：645-648

Transformation of Potted Gerbera jamesonii Bolus Using Particle Bombardment

Bi-Zeng Mao*, Lan-Lan Shan , Wei-Wei Fan , Li-Han Zhao, De-Bao Li

（Biotechnology Institute, Zhejiang University, Hangzhou 310029, China）

Abstract: The in vitro regeneration and transformation of potted Gerbera jamesonii Bolus were investigated. The effects of hormone and hormone combination on the adventitious buds proliferation and target distance on the transformation frequency were studied. The results indicated that 1.5 mg/L BA significantly increased adventitious buds proliferation to 8.96-fold than that of hormone free treatment. 9 cm target distance significantly improved the transformation frequency, which was up to 10.9%.

Key words: potted Gerbera jamesonii Bolus; regeneration system; particle bombardment; transformation system; transgenic plants

*Corresponding author. Tel: 86-571-86971678, E-mail: maobz@zju.edu.cn