Vol.26 No.6£¨2004 December£©£º631-634 The Relationship of the Rabbit Somatic Donor Cells at Different Cell Cycle and the Development of Nuclear-transferred Embryos Ru Hao, Yu-Ge Wang1, Zheng-Wang Zhang, Miao Du1* £¨College of Life Sciences, Beijing Normal University, Beijing 100875, China; 1Institute of Genetic and Developmental Biology, Chinese Academia Science, Beijing 100080, China£© Abstract: Cell cycle of rabbit fetal fibroblast cells treated with or without serum starvation in vitro was analyzed by flow cytometer and nuclear-transferred embryos were examined in vitro and in vivo. Serum starvation could remarkably increase the percentages of G0/G1 stages from 73.2% in normal condition (containing 10% FCS) to above 86%, but there were no significant difference among serum starvation for 1, 2 and 3 days. Donor cells treated with different culture time were fused into enucleated oocytes. And the fusion rate between donor cell and enucleated oocyte, division rate of reconstructed embryos cultured in vitro and implantation rate of reconstructed embryos in vivo were compared, in which showed that: serum starvation could significantly increase the fusion rate, division rate and embryo implantation rate; donor cells starved for 1 day or more could largely increase the fusion rate and division percentage, starved for 1 days could increase the reconstructed embryo implantation rate in vivo. Key words: fetal fibroblast cells; somatic nuclear transfer; donor cells; serum starvation; rabbit *Corresponding author. Tel: 86-10-62555140, E-mail: dumiao@263.net |