Vol.26 No.6£¨2004 December£©£º623-626 Mitosis in Primary Cultures of Silkworm Yu-Bin Ding, Min-Hui Pan, Shi-Quan Xiao, Min Chen, Xi-Jun Hong, Cheng Lu* £¨The Key Sericulture Laboratory of Agriculture Ministry, College of Sericulture and Biotechnology, Southwest Agriculture University, Chongqing 400716, China£© Abstract: The explants used for primary culture are tissue and semi-digestive tissue, which originate from the late embryonic tissue of silkworm (Bombyx mori). The optimal conditions for primary culture are Grace's medium supplemented with moderate amount of antibiotics, 20% fetal bovine serum and incubated at temperature 28 ¡æ, pH 6.4-6.8. The whole process of mitosis and abnormal mitosis such as abnormal cell division plate and endonuclear mitosis were found when tissue had been cultured more than 6 months. With the time of culture prolonged, divide ratio increased from 0.02¡À0.01 to 0.35¡À0.10, while the percentage of abnormal mitosis decreased from 8.0¡À1.6 to 3.2¡À1.0. Key words: Bombyx mori; primary cell; mitosis *Corresponding author. Tel: 86-23-68250346, Fax: 86-23-68251128, E-mail: lucheng@swau.cq.cn |