Vol.26 No.5£¨2004 October£©£º548-549 A Simple Method for DNA Extraction from Human Blood Clot Li Chen*, Li Jiang, Yang Luo, Xue Zhang £¨The Research Center for Medical Genomics and Key Laboratory of Cell Biology Affiliated to Ministry of Public Health of China, China Medical University, Shenyang 110001, China£© Abstract: The method described here enables to extract DNA from human blood clots. The key procedures included: blood clots were homogenized with 80 g/L NaCl and 10% EDTA, the homogenized samples were lysed with 500 ml of digestion buffer (10 mmol/L Tris, 1 mmol/L EDTA, 20 g/L SDS, 300 mg/ml proteinase K) and incubated for 2 h at 56 ¡æ, then the homogenized samples were incubated overnight at 37 ¡æ. This method was reliable for obtaining high quantities of DNA suited for PCR and restriction digest. Key words: blood clot; DNA extraction; PCR *Corresponding author. Tel: 86-24-23256666-5532, Fax: 86-24-23265539, E-mail: chenlisy@yahoo.com |