Vol.26 No.4（2004 August）：339-343

RNAi and the Stem Cell

Fei-Xue Li, Yan-Ling Wang*

（State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China）

Abstract: cDNA array, gene chip, iAFLP (introduced AFLP), SAGE (serial analysis of gene expression), cDNA-AFLP, DDRT-PCR, RC4D (RFLP-coupled differential display) and SSH (suppression subtractive hybridization) are techniques being used extensively to identify differentially regulated genes in organisms. In this review, these display systems are evaluated and compared. The general principles on which the different techniques are based and which determine their potential and their limitations are described. Some examples are given about the utilization of these methods.

Key words: differentially expressed gene; high throughput Screening; SAGE; DDRT-PCR; SSH

*Corresponding author. Tel: 86-10-62631832, Fax: 86-10-62565689, E-mail: wangyl@panda.ioz.ac.cn